Count barcodes using guide-counter
Usage
count_bc(tdir, all_constructs, valid_barcodes, reverse_complement = FALSE)
Arguments
- tdir
Path to the directory with demultiplexed FASTQ files
- all_constructs
A named list of all construct libraries
- valid_barcodes
A character vector of all possible barcodes
- reverse_complement
Whether to count the reverse complement of the barcodes instead
Value
A `SummarizedExperiment` object with counts and metadata